Journal: Immunity

Article Title: Signaling through the inhibitory Fc receptor FcγRIIB induces CD8 T cell apoptosis to limit T cell immunity

doi: 10.1016/j.immuni.2019.12.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Dr. Mark Cragg InVivoMAb anti-mouse CD16/32 (clone 2.4G2) BioXcell Cat# BE0307, RRID:AB_2736987 Bacterial and Virus Strains Biological Samples Emory Transplant Center Biorepository Emory IRB protocol #00046593 Chemicals, Peptides, and Recombinant Proteins GolgiPlug BD Biosciences Cat# 555029 OVA peptide 257–264 (SIINFEKL) GenScript Cat# RP10611 CTLA-4Ig (abatacept) Bristol-Myers Squibb, Abatacept (Orencia) Anti-CD28 domain antibodies Bristol-Myers Squibb Collagenase (type 1A) Sigma-Aldrich C2654 Hyaluronidase (type 1) Sigma-Aldrich H3506 Collagenase P Sigma-Aldrich Ref# 11213865001 Collagenase D Sigma-Aldrich Ref# 1088866001 Recombinant Fgl2 R&D Systems R&D Cat# 5257-FL-050 Critical Commercial Assays MACS CD8a+ T cell isolation kit, mouse Miltenyi Biotec 130-104-075 Quick-RNA MicroPrep Kit Zyma Research R1051 SMART-seq v4 cDNA synthesis kit Takara Cat# 634894 NexteraXT kit Illumina FC-131–1096 Mouse Fgl2 ELISA Biolegend Biolegend Cat# 437807 RNeasy Plus Micro Kit Qiagen Qiagen Cat# 74034 High Capacity cDNA reverse transcription kit ThermoFisher ThermoFisher Cat# 4368814 HT HG-U133 Plus PM BeadChip Affymetrix Thermo Fisher Cat# 901261 Lightning Link R-PE Antibody Labeling kit Novus Biologicals Cat # 703–0010 CountBright Beads Life Technologies Cat # C36950 Deposited Data RNAseq data NCBI Gene Expression Omnibus (GEO) GSE118439 Experimental Models: Cell Lines B16 Melanoma-OVA Brown, et al., 2001 , provided by Dr. Yang-Xin Fu Experimental Models: Organisms/Strains NCI C57Bl/6Ncr (NCI grantee program) Charles River Stock #556 NCI B6-LY5.1/Cr (NCI grantee program) Charles River Stock #564 OT-I Hogquist et al., 1994 OT-II Barnden et al., 1998 mOVA (C57Bl/6 background, H-2b) Ehst et al., 2013 Dr. Marc Jenkins B6.129P2-Aicda tm1(cre)Mnz /J ( Aicda −/− ) The Jackson Laboratory Stock #007770 B6;129S-Fcgr2btm1Ttk/J ( Fcgr2b −/− ) The Jackson Laboratory Stock #002848 EM:06078 Fcgr2b Fcgr2bB6null B6(Cg)-Fcgr2btm12Sjv/Cnbc ( Fcgr2b −/− ) European Mutant Mouse Archive; Dr. J.S.

Techniques: Control, Virus, Recombinant, Cell Isolation, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Antibody Labeling, Gene Expression, Mutagenesis, Software

Journal: STAR Protocols

Article Title: High-throughput mass cytometry staining for deep phenotyping of human natural killer cells

doi: 10.1016/j.xpro.2022.101768

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Article Snippet: Maxpar® X8 Antibody Labeling Kit, 158Gd , Fluidigm , Cat#201158A.

Techniques: Recombinant, Blocking Assay, Staining, Lysis, Software, Antibody Labeling, Sterility, Microscopy, Spectrophotometry

Journal: STAR Protocols

Article Title: Murine brain tumor microenvironment immunophenotyping using mass cytometry

doi: 10.1016/j.xpro.2022.101357

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Article Snippet: Maxpar® X8 Antibody Labeling Kit, 148Nd—4 Rxn , Fluidigm , SKU 201148A.

Techniques: Purification, Recombinant, Red Blood Cell Lysis, Centrifugation, Staining, Electron Microscopy, Antibody Labeling, Software, Sterility, Spectrophotometry

Journal: Cell reports

Article Title: T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection

doi: 10.1016/j.celrep.2019.12.027

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Article Snippet: Metal-Antibody Conjugation Nd145 (CyTOF) , Fluidigm , Cat#201145A.

Techniques: In Vitro, Blocking Assay, Multiplex Assay, Virus, Infection, Recombinant, Next-Generation Sequencing, RNA Sequencing, Software, Microscopy, Imaging, Conjugation Assay, Staining

Journal: STAR Protocols

Article Title: CyTOF protocol for immune monitoring of solid tumors from mouse models

doi: 10.1016/j.xpro.2022.101949

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Article Snippet: Maxpar MCP9 Antibody Labeling Kit, 112Cd—4 Rxn , Standard BioTools , 201112A.

Techniques: Blocking Assay, Purification, Recombinant, Staining, Sterility, Antibody Labeling, Software, Transferring, Microscopy

Journal: Cell reports

Article Title: T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection

doi: 10.1016/j.celrep.2019.12.027

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Article Snippet: Metal-Antibody Conjugation Er170 (CyTOF) , Fluidigm , Cat#201170A.

Techniques: In Vitro, Blocking Assay, Multiplex Assay, Virus, Infection, Recombinant, Next-Generation Sequencing, RNA Sequencing, Software, Microscopy, Imaging, Conjugation Assay, Staining

Journal: Cell

Article Title: Single-cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment

doi: 10.1016/j.cell.2018.05.060

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: N/A Microfluidic chips and consumables Droplet Genomics https://dropletgenomics.com Chromium Single Cell 5’ Library and Bead Kit 10× Genomics cat no. 1000014 HiSeq 2500 Illumina at MSKCC N/A NEBNext mRNA Second Strand Synthesis Module NEB cat no. E6111S HiScribe T7 High Yield RNA Synthesis Kit NEB cat no. E2040S Kapa 2× HiFi HotStart PCR mix Kapa Biosystems cat no. KK2601 CyTOF2 Fluidigm N/A CyTOF2 Supersampler & Sample Chiller Victorian Airship & Scientific Apparatus N/A Indium(III) Chloride Sigma Cat: 203440 Maxpar X8 Antibody Labeling Kit 150Nd Fluidigm Cat: 201150A Maxpar X8 Antibody Labeling Kit 155Gd Fluidigm Cat: 201155A Maxpar X8 Antibody Labeling Kit 166Er Fluidigm Cat: 201166A Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat: 201060 Cell-ID Intercalator-Ir Fluidigm Cat: 201192 Cell-ID Cisplatin Fluidigm Cat: 201064 FoxP3 Transcription Factor Fix/Perm eBiosciences Cat: 00-5523-00 TruStain FcX BioLegend Cat: 422302 Deposited Data inDrop single-cell RNA-seq dataset GEO Accession numbers {"type":"entrez-geo","attrs":{"text":"GSE114727","term_id":"114727"}} GSE114727 , {"type":"entrez-geo","attrs":{"text":"GSE114725","term_id":"114725"}} GSE114725 10× paired single-cell TCR and RNA-seq dataset GEO Accession numbers {"type":"entrez-geo","attrs":{"text":"GSE114727","term_id":"114727"}} GSE114727 , {"type":"entrez-geo","attrs":{"text":"GSE114724","term_id":"114724"}} GSE114724 CyTOF dataset Flow Repository https://flowrepository.org/id/FR-FCM-ZYJP Software and Algorithms SEQC This paper https://github.com/ambrosejcarr/seqc.git Biscuit This paper and Prabhkaran et al., 2016 . https://github.com/sandhya212/BISCUIT_SingleCell_IMM_ICML_2016 t-SNE van der Maaten and Hinton, 2008 . https://lvdmaaten.github.io/software/ PhenoGraph Levine et al., 2015 . https://github.com/jacoblevine/PhenoGraph Diffusion maps Coifman et al., 2005 . http://www.math.jhu.edu/~mauro/#tab_DiffusionGeom FACSDiva BD Biosciences RRID:SCR_001456 FlowJo BD Biosciences RRID:SCR_008520 https://www.flowjo.com/ FastQC Andrews, 2010. https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ STAR 2.5.3a Dobin et al., 2013. https://github.com/alexdobin/STAR Edittag Faircloth and Glenn, 2012. https://github.com/faircloth-lab/edittag Cell Ranger 2.1.1 10× Genomics https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest Loupe Cell Browser 10× Genomics https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest#loupetab CyTOF cell debarcoder Zunder et al., 2015. https://github.com/nolanlab/single-cell-debarcoder CyTOF Bead Normalization Finck et al., 2013. https://github.com/nolanlab/bead-normalization/ Open in a separate window KEY RESOURCES TABLE

Techniques: Staining, Antibody Labeling, Software, Diffusion-based Assay

Journal: STAR Protocols

Article Title: CyTOF protocol for immune monitoring of solid tumors from mouse models

doi: 10.1016/j.xpro.2022.101949

Figure Lengend Snippet:

Article Snippet: Maxpar® X8 Antibody Labeling Kit, 167Er—4 Rxn , Standard BioTools , 201167A.

Techniques: Blocking Assay, Purification, Recombinant, Staining, Sterility, Antibody Labeling, Software, Transferring, Microscopy